01N09PL2

1. 01N09PL2 is a 63 plant population derived from a strain cross of multiple Pioneer breeding lines. These parent lines were phenotypically selected for stem nematode and Northern root-knot nematode as well as for one or more of the following: anthracnose race 1, Phytophthora root rot, Aphanomyces root rot (races 1 and 2), bacterial wilt, Fusarium wilt, Verticillium wilt, and spotted alfalfa aphid. Parent plants were also selected phenotypically for field vigor, field appearance, and fall dormancy.

2. 01N09PL2 area of probable adaptation is the North Central, East Central, Moderately Winterhardy Intermountain, Winterhardy Intermountain, Great Plains regions of the United States and Ontario, Canada with the primary purpose being hay, haylage, greenchop or dehydration. 01N09PL2 has been tested for yield in Iowa, Illinois, Minnesota, Washington, Wisconsin and Ontario Canada.

3. 01N09PL2 is a dormant cultivar with fall dormancy similar to FD-4 check. Flower color in the Syn 2 generation is 92% purple, 8% variegated, with traces of yellow, white and cream found.

4. 01N09PL2 is highly resistant to anthracnose (Race 1), bacterial wilt, Verticillium wilt, Phytophthora root rot, pea aphid, stem nematode, and Northern root-knot nematode; resistant to Fusarium wilt, spotted alfalfa aphid and Aphanomyces root rot (Race 1); moderate resistance to Aphanomyces root rot (Race 2). 01N09PL2 has not been tested for blue alfalfa aphid resistance.

5. Breeder seed (Syn 2) was produced from 248 random plants started as seedlings in the greenhouse, and transplanted to cage in the Pacific Northwest during the summer of 2001. Seed classes will be breeder (Syn 2), foundation (Syn 2 or 3), and certified (Syn 3, 4, or 5). Foundation seed may be produced from breeder or foundation. The second generation foundation seed may be produced at the discretion of Pioneer Hi-Bred International, Inc. Limitations of age of stand will be one, three and five years, respectively, for breeder, foundation and certified seed. Pioneer Hi-Bred International will maintain breeder seed for the life of the cultivar.

6. Seed will be marketed in the spring of 2007.

7. Application for Plant Variety Protection may be made and the certification option will not be requested.

8. As a means of added varietal protection, information included with the Application for Review of Alfalfa Varieties for Certification may be provided to the PVP office.

9. Variety name: 01N09PL2 Date submitted: November 30, 2005

Experimental designation: N00SL78

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