ZN 9646

1. ZN 9646 was selected for resistance to the following pests: bacterialwilt, Fusariurn wilt,Verticillium wilt, Phytophthora root rot, anthracnose(race 1) and Aphanomyces (race 1) plustwo additional isolates. Phenotypicrecurrent selection was used. Final selections were madefrom two-year oldspace plant selection nurseries near Napier, Iowa, Livingston and Marshfield,Wisconsin, based on yield, winter survival, degree of leafhopper yellowingand stunting,freedom from leaf diseases, fall dormancy reaction and stemprotein.

Breeder seed (syn1) was produced in 1996 on approximately 25 cuttingsof each clonetransplanted at random and harvested in bulk.

2. ZN 9646 appears to be adapted to the north central, east central andGreat Plains regions ofthe U.S. It is intended for use in the north central,east central and Great Plains regions of theU.S. It has been tested in Iowa,Illinois, Indiana, Wisconsin, Pennsylvania, and Kansas.

3. Fall dormancy of ZN 9646 is similar to the FD 4 check Saranac. Flowercolor of syn2generation is approximately 72% purple and 28% variegated witha trace of cream, yellow andwhite.

4. ZN 9646 has high resistance to bacterial wilt, Verticillium wilt, Fusariurnwilt,anthracnose(race 1), Phytophthora root rot, pea aphid, and Aphanomyces(race 1). It hasmoderate resistance to blue alfalfa aphid. It has not beentested for reaction to spotted alfalfaaphid, stem nematode or root knot nematode.

5. Seed increase is limited to one generation each of breeder (syn1),foundation (syn2) andcertified (syn.3) seed classes. Certified seed may beproduced from either breeder or foundationclasses. A one, three and six yearstand life is permitted on fields producing breeder, foundationand certifiedseed classes respectively. Foundation seed production is limited to the PacificNorthwest. Breeder seed was produced in 1996. ABI will maintain sufficientseed stocks for thelife of the variety.

6. Certified seed will be available in 2000.

7. Application will not be made for Plant Variety Protection.

8. This information can be forwarded to the PVP office.

9. Date submitted: November 1999

Experimental designations: ZN 9646

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