TMF421 1. TMF 421 is a synthetic variety with 107 parent plants which were selected simultaneously for resistance to Phytophthora root rot and Aphanomyces root rot (race 1). Parent plants were selected from crosses between selections from various populations in three year old Wisconsin nurseries. Source populations were developed by phenotypic recurrent selection for high relative feed value (using Near Infrared Reflectance Spectroscopy), and for resistance to one or more of the following pests: bacterial wilt, Verticillium wilt, Phytophthora root rot, Aphanomyces root rot (race 1), anthracnose (race 1), and Leptosphaerulina leafspot Parentage of ThlF 421 traces to the following germplasm sources: DK 122, GH 767, and Pacesetter. Approximate germplasm source contributions are as follows: M. falcata (9%), Ladak (6%), M. varia (24%), Turkistan (3%), Flemish (48%), and Chilean (10%). 2. TMF 421 is adapted to the North Central U. S. and is intended for use in the northern and central U. S. . TMF 421 has been tested in Wisconsin and Minnesota. 3. TMF 421 is a dormant variety with fall dormancy similar to Vernal. Flower color observed in the Syn. 2 generation is approximately 94% purple, 5% variegated, 1% cream, and a trace of white and yellow. Forage quality of TMF 421 is similar to WL322HQ. 4. TMF 421 has high resistance to bacterial wilt, Verticillium wilt, anthracnose (race 1), Phytophthora root rot, Aphanomyces root rot (race 1), pea aphid, and stem nematode, with resistance to Fusarium wilt and spotted alfalfa aphid. Reaction to root knot nematode and blue alfalfa aphid has not been adequately tested 5. Seed increase of TMF 421 is on a limited generation basis with two generations of the breeder, foundation, and certified seed classes. Breeder (Syn. 1 or Syn. 2), foundation (Syn. 2 or Syn. 3), and certified (Syn. 3 or Syn. 4) classes will be recognized. Production of Syn. 2 breeder or Syn. 3 foundation seed requires the consent of the breeder. Breeder seed was produced under cage isolation at Woodland, California in 1992. Sufficient foundation seed for the projected life of the variety will be maintained by Cal/West Seeds. Stands of foundation and certified seed fields are limited to 3 and 6 years, respectively. 6. Certified seed of TMF 421 will be available in 1996. 7. No decision has been made regarding Plant Variety Protection. 8. This information can be forwarded to the PVP office. 9. Variety Name: TMF 421 Experimental Designation: CW 2227 Date NA & MLVRB first accepted this variety: January1996 Previous Page