329(amended)

1. 329 is a synthetic variety with 218 parent plants which were selected sequentially for multifoliolate leaf expression and for resistance to Phytophthora root rot and Aphanomyces root rot (race 1). Parent plants were selected from various populations that were developed by phenotypic recurrent selection for high relative feed value (using Near Infrared Reflectance Spectroscopy), for multifoliolate leaf expression, and for resistance to one or more of the following pests: bacterial wily Aphanomyces root rot (race 1),Phytophthora root rot, anthracnose (race 1),Verticillium wilt, Leptosphaerulina leafspot and spotted alfalfa aphid. Parentage of 329 traces to the following germplasm sources: Class, Alfaleaf, DK 122,Crown II, Encore, Prism, Achieva, DK 133, VS-775, and Precedent. Approximate germplasm source contributions are as follows: M. falcata (8%), Ladak (6%), M. varia (25%), Turkistan (5%), Flemish (47%), and Chilean (9%).

2. 329 is adapted to the northcentral and northeastern U.S. and is intended for use in the northern and central U.S.. 329 has been tested in Wisconsin, Minnesota and Pennsylvania.

3. 329 is a dormant variety with fall dormancy similar to Ranger. f Flower color observed in the Syn.2 generation is approximately 89% purple, 11% variegated, and a trace of white, cream, and yellow. At first flower in autumn approximately 77% of the stems show multifoliolate leaf expression.

4. 329 has high resistance to bacterial wilt, Fusarium wilt, Verticillium wilt, anthracnose (race 1), Phytophthora root rot and pea aphid, and resistance to Aphanomyces root rot (race 1), stem nematode,and spotted alfalfa aphid. Reaction to root knot nematode, and blue alfalfa aphid has not been adequately tested.

5. Seed increase of 329 is on a limited generation basis with one generation of breeder and two generations of the foundation and certified seed classes. Breeder (Syn. l), foundation (Syn.2 or Syn.3), and certified (Syn.3 or Syn.4)classes will be recognized. Production of Syn.3 foundation seed requires the consent of the breeder. Breeder seed was produced under cage isolation at Woodland, California in 1991. Sufficient foundation seed for the projected lifeof the variety will be maintained by Cal/West Seeds. Stands of foundation and certified seed fields are limited to 3and 6 years, respectively.

6. Certified seed of 329 will be available in 1994.

7. No decision has been made regarding Plant Variety Protection.

8. This information can be forwarded to the PVP office.

9. Variety Name: 329. Experimental Designation: CW 1312. Date NA&MLVRB first accepted this variety: January 1994. Dates previous amendments were accepted: January 1996. Date this amendment submitted: December 1, 1996.