Abstracts for the
38th North American
Alfalfa Improvement Conference
July 27 - 31, 2002
Sacramento, CA
NAAIC Pre-Conference Workshop
Medicago truncatula: A Model Medicago Species for Molecular Biology.
University of California, Davis
Saturday, July 27
Moderator: Gary Bauchan, USDA-ARS, Beltsville, MD
8:15am Introduction
9:00am The organization of the genetic diversity in the complex of species M. truncatula - M. littoralis. Jean-Marie Prosperi, INRA, Mauguio, France.
9:30am Regeneration, transformation and insertion mutagenesis in M. truncatula. Pascal Ratet, CNRS, Gif-sur-Yvette, France.
10:00am
Break
10:15am Gene Mapping. The genetics and genomics of Medicago. Gyorgy Kiss, University of California, Davis, CAA
10:45am
Transcription analysis of pathogen
interactions with M. truncatula using microarrays. Debby Samac, USDA-ARS,
St. Paul, MN
11:15am The Medicago truncatula genome initiative. Gregory May, Noble Foundation, Ardmore, OK.
11:45am Medicago truncatula EST-SSRs for genetic mapping in autotetraploid alfalfa. Mary Sledge1, M. Mian1, and Ian Ray2, 1Noble Foundation, Ardmore, OK and 2New Mexico State University, Las Cruces, NM
12:15pm Lunch
1:30pm BAC-based genetic, cytogenetic and physical map of M. truncatula. Dong-Jin Kim, University of California, Davis, CA.
2:00pm Genome wide identification of nodule specific transcripts in Medicago truncatula. M. Fedorova1, J. van de Mortel1, P. Matsumoto1, J. Cho2, C. D. Town2, K. A. VandenBosch3, J. S. Gantt3, C. P. Vance1,4 1Department of Agronomy and Plant Genetics, Univ. Minnesota, St. Paul, MN 2The Institute for Genomic Research, Rockville, MD 3Department of Plant Biology, Univ. Minnesota, St. Paul, MN 4USDA-ARS-Plant Science Research, St. Paul, MN
2:30pm Resources for gene expression analysis in M. truncatula, and their use for profiling symbiotic development. Kathryn VandenBosch, University of Minnesota, St. Paul, MN.
3:00pm Break
3:15pm Pathosystems and resistance gene genomics in M. truncatula. Doug Cook, University of California, Davis, CA.
3:45pm Use of M. truncatula EST collections to isolate glucosidases hydrolyzing isoflavonoid 7-O-glucose conjugates. Nancy Paiva, C. Mei, J.D. Cooper, and L.A. Riner. Noble Foundation, Ardmore, OK.
4:15pm Comparative
analysis of plant growth and development: use of M. truncatula as a bridge
species. Varma Penmetsa, University of California, Davis, CA.
2002 NAAIC Conference
University of California, Davis
Monday, July 29
Symposium: Medicago Genomics & Biotechnology
Moderator: Joe Bouton, NAAIC President, University of Georgia, Athens, GA.
8:00am Introductions
8:15am Breeding alfalfa in the genomics era: WeĠre moving faster, but where are we going? Charlie Brummer, Iowa State University, Ames, IA
9:00am Challenges and opportunities in commercialization of transgenic traits in alfalfa. Mark McCaslin, Forage Genetics, Prior Lake, MN
9:45am Development of alfalfa as a biofactory for the commercial production of recombinant molecules. Pierre Bilodeau, Medicago, Inc. Ste. Foy, Quebec, Canada
10:15am Break
10:30am Medicago trancatula genome initiative. Gregory May, Nobel Foundation, Ardmore, OK
11:15am Genetic, genomic and molecular prospecting in Medicago truncatula for improvement of crop legume species. Doug Cook, University of California, Davis, CA.
Monday, July 29, 1:00pm
Breeding and Genetics
Moderator: Jonathan Reich, Cal-West Seeds, Woodland, CA.
1:00pm Marker-assisted backcrossing of aluminum tolerance QTL from diploid Medicago sativa subsp. coerulea into tetraploid M. sativa subsp. sativa. Mary K. Sledge and Joe H. Bouton. Noble Foundation, Ardmore OK and University of Georgia, Athens, GA.
1:20pm Alfalfa, cold stress, and a heat shock transcription factor. Jeremy N. Friedberg and Stephen R. Bowley. University of Guelph, Guelph, Ontario, Canada
1:40pm Cytogenetic investigations of non-dormant alfalfa germplasm sources. Gary R. Bauchan, T. Austin Campbell, and M. Azhar Hossain. USDA-ARS, Beltsville, MD
2:20pm Alfalfa pollen-mediated gene flow studies, 2000-2001. S. Fitzpatrick, P. Reisen, and M. McCaslin. Forage Genetics, West Salem, WI
2:40pm Cloning the nodulation gene nnl from the tetraploid alfalfa (Medicago sativa) mutant MN1008. G. B. Kiss, Z. Kevei, P. Kalo, A. Kereszt, and G. Endre. University of California, Davis, CA
3:00pm Break
3:30pm Genetic mapping and identification of QTL for stem morphogenesis in tetraploid alfalfa. B. Julier, F. Guines, C. Hackett, C. Ecalle, and C. Huyghe. UGAPF-INRA, Lusignan, France.
3:50pm Genetic manipulation of alfalfa for organic acid synthesis and aluminum tolerance. D. Samac, M. Tesfaye, D. A. Allan, and C. P. Vance. USDA-ARS, St. Paul, MN
4:10pm Enhanced alfalfa world collection germplasm. J. L. Caddel, A. A. Zarrabi, R. C. Berberet, and J. D. Prater. Oklahoma State University, Stillwater, OK.
4:30pm Forage yield response of alfalfa to percent hybridism. Mike Velde, et al. Dairyland Research, Clinton, WI.
Monday, 7:00 - 10:00 p.m.
POSTER SESSION:
Breeding and Genetics.
1. Improving cold-tolerance of alfalfa through sucrose-phosphate synthase over expression. Heather Shearer and Stephen Bowley. University of Guelph, Guelph, Ontario, Canada
2. Screening Medicago truncatula microsatellite markers for inclusion in a cultivated alfalfa microsatellite map. T.A. Campbell1, N. Young2, T. Huguet3, and G.R. Bauchan1, 1USDA-ARS, Beltsville, MD; University of Minnesota, St. Paul, MN; CNRS-INRA, Castanet-Tolosan, France.
3. An efficient system for protoplast culture from alfalfa (Medicago sativa L.) Suitable for plant transformation and regeneration. V. Levee, M. Bertrand, M. Duval, L-P. Vezina, and P. Bilodeau. Medicago, Inc. Ste. Foy, Quebec, Canada.
4. Alfalfa wild relatives collected from northwestern Kazakistan. S. L. Greene, A. Afonin, N. I. Dzyubenko, A. Khusainov, and R. Hannan. USDA-ARS, Prosser, WA.
5. Heterotic performance of a broad range of sativa-falcata hybrids. H. Riday and E. C. Brummer. Iowa State University, Ames, IA.
6. Relationship between seed yield and forage yield in alfalfa. Joseph G. Robins and E. Charles Brummer. Iowa State University, Ames, IA
7. Towards alfalfa free-hybrids. Diallel crosses among the free hybrid parental constituents (2S2Syn3 and DH F2): Pod fertility. P. Rotili, C. Scotti, S. Gnocchi, D. Kertikova, and G. Gnocchi. Istituto Sperimentale Cotture, Lodi, Italy.
8. Breeding for persistence of alfalfa (Medicago sativa L.) varieties. Bela Nagy. Gate ÒFleischmann Rudolf, Kompolt, Hungary.
9. Strategies for alfalfa plastid transformation. Pieriuigi Barone1, Daniele Rosellini1, Fabio Veronesi1, Peter LaFayette2, Joseph Bouton2, and W. A. Parrott2. 1Instituto di Miglioramento Genetico Vegetale, Perugia, Italy and 2University of Georgia, Athens, GA.
10. Genetic variability and heritability of salt tolerance in alfalfa (Medicago sativa L.). M. Hefny and R. Dolinski. Suez Canal University, Ismaila, Egypt.
11. Inferences from mitochondrial DNA patterns on the domestication history of alfalfa (Medicago sativa). Marie-Helene Muller, Joelle Ronfort, Sylvain Santoni, and Jean-Marie Prosperi. INRA, Mauguio, France.
12. Consequences of domestication bottleneck on the DNA sequence diversity of a Pectate Lyase Analog gene in wild and cultivated Medicago sativa. Marie-Helene Muller, Joelle Ronfort, Sylvain Santoni, and Jean-Marie Prosperi. INRA, Mauguio, France.
13. Aluminum tolerance in alfalfa with the citrate synthetase gene. Daniele Rosellini1, Joe Bouton2, Pete LaFayette2, Mary Sledge3, Fabio Veronesi1, and Wayne Parrott2. 1Instituto di Miglioramento Genetico Vegetale, Perugia, Italy; 2University of Georgia, Athens, GA; and Noble Foundation, Ardmore, OK.
14. Maximizing expression of transgenic traits in autopolyploid plants. Stephen J. Temple, Bruce J. Drummond, Jessica E. Tofte, and Mark McCaslin. Forage Genetics, West Salem, WI.
Entomology and Plant Pathology
15. Genetic variation in alfalfa for inhibition of lygus (Lygus hesparus L.) salivary enzymes. L. R. Teuber, M. P. Celorio-Mancera, K. L. Taggard, L. C. Greve, K. A. Shackel, H. Ahmadi, and J. M. Labavitch. University of California, Davis, CA.
16. Alfalfa persistence under alfalfa weevil pressure. V. N. Owens and M. A. Catangui. South Dakota State University, Brookings, SD.
17. Within-plant spatial patterns and preferred feeding sites of cowpea aphids on alfalfa. Ali A. Zarrabi, Richard C. Berberet, and Mark E. Payton. Oklahoma State University, Stillwater, OK.
18. Trends about alfalfa resistance to the pea aphid in France. Rene Bournoville. INRA, Lusignan, France.
19. Visualizing early events of pathogenesis of Phoma medicaginis var. medicaginis on alfalfa (Medicago sativa L.). C. Castell and D. Samac. University of Minnesota, St. Paul, MN and USDA-ARS, St. Paul, MN.
20A. Molecular variability and pathogenesis of Phoma medicaginis var. medicaginis isolates from Minnesota. C. Castell, D. Samac, and L. J. Szabo. University of Minnesota, St. Paul, MN and USDA-ARS, St. Paul, MN
20B. Pathosystem Development and Transcriptome Analysis of Pathogen Interactions with Medicago truncatula. D. A. Samac1,2, S. Peñuela2, D. Danesh2, N. D. Young2, and K. VandenBosch3 1USDA-ARS-Plant Science Research, St. Paul, MN 2Department of Plant Pathology, Univ. Minnesota, St. Paul, MN 3Department of Plant Biology, Univ. Minnesota, St. Paul, MN
Physiology, Quality, Utilization
21. Alfalfa seed production for domestic market and for export in Egypt. Mohamed A. Z El-Nahrawy. Field Crops Institue, Giza, Egypt.
22. Dodder control in Roundup Ready alfalfa. Peter Reisen, Mark McCaslin, and Nolan Johannsen. Forage Genetics, Nampa, ID
23. Withdrawn.
24. Competitive ability of various lucerne (Medicago sativa L.) varieties to stimulate biological nitrogen fixation. M. M. Mohamed, Z Staszewski, and A. Rammah. Institute of Plant Breeding, Blonie, Poland.
25. Comparison of North American alfalfa varieties productivity in Poland. Z. Staszewski and Z. Bodzon. Institute of Plant Breeding, Blonie, Poland.
26. Breeding alfalfa for southern Australian dryland farming systems. A. W. Humphries1, G. C. Auricht1, and R. Latta2. 1South Australian Research and Development Institute, Adelaide, South Australia and 2Agriculture Western Australia, Katinning, Western Australia, Australia.
27. The effect of waterlogging on photosynthetic parameters in lucerne. C. Smethurst, T. Garnett, G. Auricht, and S. Shabala. South Australian Research and Development Institute, Adelaide, South Australia, Australia.
28. Comparison of seed and seedling vigour in alfalfa with fenugreek, sweet clovers, berseem clovers, and Persian clover. R. N. Arora. C.C.S. Haryana Agricultural University, Haryana, India
29. Effect
of mevalonate kinase gene on stem growth in transgenic alfalfa. B. Julier,
M. Czochara, V. Lauvergeat, and C. Huyghe.
30. Effect of condensed tannins on protein solubility of alfalfa. B. Julier, J. C. Emile, P. Morris, G. Allison, M. Robbins, and C. Huyghe
31. Principal Pollinators of Alfalfa in South Russia. M.I.Voloshin. Agro-industrial Scientific System “Medita”, Krasnodar, Russia
32. Characterization of Genes Expressed During the Early Stages of Direct Somatic Embryogenesis in Alfalfa (Medicago falcata L.). Yan Zhou, Mark R Fowler, Jenny Russinova, Cui-Ying Shao, Nigel W Scott, Malcolm C Elliott and A Slater. The Norman Borlaug Institute for Plant Science Research, De Montfort University, Leicester, UK
33. Grazing Tolerance of Crioula Alfalfa in Southern Brazil. Naylor B. Perez; Rogério J. Santos; Thiago Barros; Miguel DallAgnol. UFRGS, Faculdade de Agronomia, Porto Alegre, RS
Tuesday, July 30, 8:30am
Entomology and Plant Pathology
Moderator: Landon Rhodes, Ohio State University, Columbus, OH
8:30am Expression of a fungal medicarpin detoxification gene in alfalfa increases susceptibility to Phoma medicagenis. N. L. Paiva, S. R. Mundodi, and J. D. Cooper. Noble Foundation, Ardmore, OK.
8:50am Effects of potato leafhopper injury and Fusarium crown rot on three alfalfa populations. Jennifer J. Ariss, L. H. Rhodes, R. M. Sulc, and R. B. Hammond. Ohio State University, Columbus, OH
9:10am Quantifying soilborne pathogens in alfalfa with fluorescent polymerase chain reaction assays. George J. Vandemark and Bridget M. Baker. USDA-ARS, Prosser, WA.
9:30am Cold tolerance in adult parasites attacking the alfalfa weevil. Richard C. Berberet, A. D. Bisges, and Ali A. Zarrabi. Oklahoma State University, Stillwater, OK.
9:50am Evaluation of alfalfa and birdsfoot trefoil on alfalfa snout beetle infested fields. J. Hansen, J.Miller-Garvin, and D. Viands. Cornell University, Ithaca, NY
1010am Break
10:40am Reaction of twenty-one alfalfa cultivars to the lesion nematode Pratylenchus penetrans. S. L. Hafez1, P. Sundararaj1, and D. Miller2. 1University of Idaho, Parma, ID and 2ABI Alfalfa, Nampa, ID.
11:00am Simulating lygus bug damage with micro-injection. K. A. Shackel, H. Ahmadi, L. C. Greve, M. P. Celorio-Mancera, J. M. Labavitch, and L. R. Teuber. University of California, Davis, CA.
11:20am Digestive enzymes in lygus bug saliva and extracts: A preliminary characterization. M. P. Celorio-Mancera, L. C. Greve, K. A. Shackel, L. R. Teuber, H. Ahmadi, and J. M. Labavitch. University of California, Davis, CA.
11:40am Plant parasitic nematodes of alfalfa in the United States. F. A. Gray and G. D. Griffin. University of Wyoming, Laramie, WY.
Physiology, Quality, Utilization
Moderator: Dennis Rowe, USDA-ARS, Mississippi State, MS.
8:00am Effect of wheel traffic on alfalfa. Jim Moutray1 and Dan Undersander2. 1ABI Alfalfa, Ames, IA and 2University of Wisconsin, Madison, WI.
8:20am Comparison of visual assessment and quantitative measurement for estimating plant persistence in the selection of grazing tolerant alfalfa in Italy and the USA. L. Pecetti1, E. Piano1, M. Romani1, and J. H. Bouton2. 1Istituto Sperimentale Cotture, Lodi, Italy and 2University of Georgia, Athens, GA.
8:40am Effect of plant age on cold acclimation and freezing tolerance of alfalfa. Y. Castonguay, P. Nadeau, and R. Michaud. Agriculture and Agri-Food Canada, Ste. Foy, Quebec, Canada.
9:00am Herbage and N production by nondormant alfalfas in Minnesota. C. C. Sheaffer, J. F. S. Lamb, and P. R. Peterson. University of Minnesota, St. Paul, MN and USDA-ARS, St. Paul, MN.
9:20am Break
9:40am Evaluation of annual medics (Medicago spp.) as living cover crop mulches in vegetable production systems in the Midwest. C. A. Wyenandt, R. M. Riedel, L. H. Rhodes, and M. A. Bennett. Ohio State University, Columbus, OH.
10:00am Fatty acid evaluation in roots and crowns of alfalfa genotypes contrasting in winter hardiness and ploidy level. B. Alarcon-Zuniga, E. C. Brummer, K, J. Moore, and P. Scott. Iowa State University, Ames, IA.
10:20am Selecting
for winter hardiness in non-dormant alfalfa. Mindy A. Weishaar and E.
Charles Brummer. Iowa State University, Ames, IA.
10:40am Improving alfalfa digestibility by genetic manipulation of lignin biosynthesis. M. McCaslin, D. Guo, S. Temple, D. Whalen, Peter Reisen, Sharie Fitzpatrick, Sam Stratton and R. A. Dixon
Wednesday, July 31, 1:30pm
Physiology, Quality, Utilization
Moderator: Gary Hoard, Pioneer Hi-Bred International, Johnston , IA.
1:30pm Alfalfa yield and quality response to molybdenum, boron, and lime applications. R. D. Meyer1, D. B. Marcum2, and S. Orloff3. 1University of California, Coop. Extension, 1Davis, CA; 2McArther, CA; and 3Yreka, CA.
1:50pm Comparing yield and forage quality of alfalfa cultivars released from the 1940's through the 1990's. JoAnn F. S. Lamb1, Craig C. Sheaffer2, Paul C. St. Amand3, E. Charles Brummer4, Landon H. Rhodes5, Marc Sulc5, and Daniel J. Undersander6. 1USDA-ARS, St. Paul, MN; 2University of Minnesota, St. Paul, MN; 3Kansas State University, Manhattan, KS; 4Iowa State University, Ames, IA; 5Ohio State University, Columbus, CA; and 6University of Wisconsin, Madison, WI.
2:10pm Cultivar and nitrogen status effects on alfalfa pasture bloat. S. D. Cash and C. D. MacDonald. Montana State University, Boseman, MT
2:30pm Characteristics of alfalfa populations selected for improved freezing tolerance. Paul Nadeau, Yves Castonguay, Serge Laberge, and Real Michaud. Agriculture and Agri-Food Canada, Ste. Foy, Quebec, Canada.
2:50pm Break
3:20pm The economics of the yield/quality tradeoff in alfalfa. S. B. Orloff, D. H. Putnam, T. OĠNeil, and S. Blank. University of California, Davis, Ca.
3:40pm Analyzing cultivar performance utilizing both yield and quality factors. D. Putnam, S. B. Orloff, and Gary Peterson. University of California, Davis, Ca.
4:00pm Using digestible fiber to determine forage quality. Dan Undersander and Dave Combs. University of Wisconsin, Madison, WI.