1. A statement of the origin and the breeding procedures used in developing the variety.

2. Area of probable adaptation and primary purpose (hay, grazing, etc.) for which this variety will be used. Report States and areas within States where the variety has been tested, and proposed areas of recommendation and merchandising.

3. Information of value to field inspectors (such as uniformity, leaf, flower characteristics, etc.), physiological characteristics, obvious disease and insect reactions, and other identifying characteristics.

4. Procedure for maintaining stock seed, seed classes to be used, a statement as to the limitations of generations that may be certified, and any other requirements or limitations necessary to maintain varietal characteristics.

5. If this variety is accepted by official certifying agencies, when will certified seed first be offered for sale?

Information Submitted by Applicant on the Above Points:

AS-13

1. Seed was collected from 13 plants chosen from source nurseries in California and Arizona for their seed production, spotted alfalfa aphid resistance, and general agronomic performance. Nine plants trace to the variety Moapa and four to Lahontan. Thirty-six seedlings were selected from each of the 13 seed lots for seedling vigor and serve as the parent stocks of AS-13.

2. Adapted throughout southwestern United States. Adapted in more northern areas than nonhardy alfalfas. Tested in Arizona and in the San Joaquin and Sacramento Valleys of California. Well suited for haying, greenchop, and de--hy operations.

3. Predominantly purple flowers, fairly uniform growth in spring and fall. Fall and winter dormancy is intermediate between Moapa and Lahontan. Upright growth. Tolerant to spotted alfalfa aphid. More resistance to downy mildew than Moapa.

4. Seed production shall be on a four-generation basis: breeder, foundation, registered and certified. Stock seed (breeder, foundation and registered classes) will be produced in California. A reserve of breeder seed harvested in 1959-60 is maintained by Farm Seed Research Corporation. Breeder seed will be released only to Ferry - Morse Seed Company for production of foundation seed. Foundation seed will be used for production of registered or certified seed. Certified seed fields will be established only with foundation or registered seed.

5. 1970.

AS-49

1. Seed was collected from 12 plants growing in source nurseries in California and Arizona. Six of the plants trace to the variety Cody, three to Zia, and three to Lahontan. The plants were chosen because of their seed production ability, spotted alfalfa aphid resistance, and general agronomic traits. From each of the 12 seed lots, 36 seedlings were selected, based upon seedling vigor. These 432 seedlings were placed at random in an isolation-crossing block and used as a source of breeder seed.

3. Predominantly purple flowers, fairly uniform growth, and semi-dormant winter growth habit in California. Growth habit is upright. Resistant to spotted alfalfa aphid.

4. Seed production of AS-49 shall be on a four-generation basis: breeder, foundation, registered, and certified. Stock seed (breeder, foundation and registered classes) will be produced in California. A reserve of breeder seed harvested in 1960-1961 from original 432 plants is maintained by Farm Seed Research Corporation. Breeder seed will only be released to Ferry - Morse Seed Company for production of foundation seed. Foundation seed will be used to produce registered or certified seed. Certified fields will be established with registered or foundation seed.

5. 1970.

Caliente

1. Caliente is a 15-clone synthetic. Ten clones were selected from experimental strain AS-10, which was constituted from African, Indian and Iraqi plant selections; five clones were selected from the variety AS-13. Selected clones were evaluated in source nursery at Bakersfield, California, and screened for spotted alfalfa aphid resistance, downy mildew reaction, seed set, and general agronomic traits.

2. Sold in the southwestern United States for greenchop, hay, and de-hy operations. It has been tested in the Imperial, San Joaquin, Sacramento and coastal valleys of California.

3. Predominantly purple flower, fairly uniform and nondormant in winter in California. Upright growth habit, resistant to spotted alfalfa aphid and tolerant to downy mildew.

4. Seed production of Caliente will be on a four-generation basis: breeder, foundation, registered and certified. Stock seed (breeder, foundation and registered classes) will be produced in California. A reserve of breeder seed harvested in 1963 from the isolation of 12 propagules from each of the 15 clones is maintained by Farm Seed Research Corporation. Breeder seed will only be released to Ferry - Morse Seed Company for the production of foundation seed. Foundation seed will be used to produce registered or certified seed. Certified seed fields will be established only with foundation or registered seed.

5. 1970.

1. The parent clones of Moapa were reconstituted in order to improve performance of the variety and to substitute two clones that were difficult to maintain. Seven parent clones of Moapa were tested in combinations with other clones of known progeny performance. Selection was based on yield, persistence, and reaction to spotted alfalfa aphid. The following nine clones were chosen as parents of Moapa 69: C904, C905, C906, C907, C908, C909, C910 from Moapa, C1026 from Mesa-Sirsa and C937 from Sonora. C1026 and C937 were also parent clones of El Unico.

2. Moapa 69 is intended for hay production in those areas of Utah, Nevada, and California where Moapa is presently adapted. Moapa 69 has been tested at Logandale in southern Nevada, Mesa in the Salt River Valley of Arizona, El Centro in the Imperial Valley of California and Davis in the Central Valley of California.

3. Purple flowers. Similar to Moapa in uniformity of growth and winter dormancy. Rapid recovery after cutting. Moapa 69 is more resistant than Moapa to biotypes ENT A, E and F of the spotted alfalfa aphid but only slightly more resistant to

ENT C.

4. Breeder seed is a composite of equal amounts of seed from each of the nine parent clones intercrossed under isolation. Seed classes are breeder, foundation and certified. Foundation seed is the first-generation seed from fields planted with breeder seed in California, Nevada and Arizona south of the 40 parallel at elevations below 2500 feet. Certified seed is the production from fields planted with either foundation or breeder seed. No other seed class or generation is to be certified. Length of stand for foundation seed production shall not exceed 5 years from date of seeding. Breeder seed will be maintained by Nevada Agricultural Experiment Station.

5. 1971.

1. Seed fields of Tempo are established by blending equal amounts of two single crosses, each produced in isolated fields containing two clones vegetatively increased. All four of Tempos parents are self-fertile. Two of the parents were selected based on diallel crosses made in the winter of 1961-1962 and trace maternally to selections from the varieties Ranger and Buffalo. The other two parents were first used in crosses made in the winter of 1962-1963 and trace to wilt resistant segregates of a Flemish variety x Vernal cross.

2. Yield data indicate favorable performance throughout the Midwest and Middle Atlantic States. It will likely be used where Vernal, Ranger, Buffalo and Saranac are currently grown.

3. Purple and blue flowers. Recovery growth, leaf shape and color are quite uniform. Less dormant than Vernal, slightly more dormant than DuPuits and similar to Saranac. Upright. Leaf color is darker green than most varieties.

4. The four parent clones will be maintained by Farmers Forage Research Cooperative at Lafayette, Indiana. Breeder seed will be produced in two isolations with two parent clones each. Seed from these two isolations will be mixed in equal proportions to establish commercial seed fields. There will be two classes of seed - breeder seed produced on the parent clones and commercial seed (certified) which will be produced in fields established from breeder seed. Both classes will be grown in Western seed producing areas.

5. Spring of 1970.

1. Parental clones selected from plants originating from F.C. numbers 24798, 32129, 32142, 32144, 32667, and one plant of unknown origin. Source nurseries of about 500 creeping-rooted or rhizomatous plants were established in fall of 1954 at Marie and Osceola, Arkansas. Fifty clones were selected and established in P.C. nursery in 1957 at Fayetteville. P.C. and S₁ seed were obtained in 1957.

A progeny performance test was established at Fayetteville and Keiser, Arkansas, in 1958 which included P.C. and S₁ progenies, parental clones, and variety checks. Spaced plants were used to evaluate clones (1958-1960). Nine parental clones were

selected on basis of P.C. and S₁ progeny performance during 3-year period.

3. Flower color is quite variable, ranges from white through shades of yellow, green and purple. Wide, low crowns and relatively small stems under Arkansas conditions. Winter dormancy in Arkansas similar to Vernal, Culver, and Rhizoma. Semi-decumbent with some variation under grazing or thin stands. Mostly erect in dense stands. Relatively short internodes and a large number of relatively small stems per crown. Good resistance to spotted alfalfa aphid, above average tolerance to leafhoppers, some resistance to flower thrips, moderate resistance to bacterial wilt, common leaf spot, and downy mildew. Pods tightly coiled. A few sickle-shaped pods occur under Arkansas conditions.

4. Breeder seed to be produced at Fayetteville, Arkansas, in an isolated spaceplanted nursery. Equal amounts of seed from each of the nine parental clones will be composited and designated breeder seed. Foundation seed to be produced from breeder seed. Certified seed to be produced from foundation seed or breeder seed. Seed classes are limited to breeder, foundation, and certified. 5. Probably in 1971. WL 306

1. Five populations of diverse breeding were subjected to three cycles of screening for pea and spotted alfalfa aphid resistance between 1963 and 1966 as follows: (1) PX seed of 4 Falcata x Vernal crosses, (2) WL 303 and 304,(3) WL 202, 210, and 211, (4) Atlantic clones and Atlantic x Flemish crosses, and (5) Misc. PX progenies with spotted alfalfa aphid resistance. From a total of more than 200,000 seedlings exposed to aphid pressure (pea and spotted independently) in the greenhouse over the 3-year period, the best 941 survivors were planted under screen cages at Bakersfield, California, in 1966 to produce experimental 303 AWR. A minimum of 78 and maximum of 246 plants were selected from each group at the conclusion of each screening cycle to produce the next generation. Following the second cycle of screening, the survivors of populations 2, 4 and 5 were inoculated with bacterial wilt organism at planting time. Only resistant and moderately resistant plants produced the seed used in the third and final screening for aphids. Each population was increased in a separate cage. Breeder seed was composited from populations 2 and 4 to comprise 50% of the total. Equal quantities of populations 1, 3 and 5 comprise the other

50%. The 21+ pounds of seed thus produced became the breeder seed as well as initial seed for testing.

2. Adapted for hay or greenchop in the Central Region of Alfalfa Adaptation. Has been tested in Kansas, Missouri, Illinois, Iowa, Nebraska, Minnesota, Ohio, and Pennsylvania.

3. Approximately 70% purple, 20% blue or bluish-green variegated, and 10% yellow, yellow variegated and white flower color. Less uniform than Buffalo or Cody but as uniform as Vernal. Slightly less fall dormant than Buffalo or Saranac. Predominantly upright but moderately decumbent under short days. Moderately variegated for an intermediate type alfalfa. Pod shape varies from tightly to loosely coiled with some sickle-shaped.

4. The initial isolated increases of 941 surviving plants from the third cycle of screening for pea and spotted alfalfa aphids comprise the breeder seed for WL 306. Foundation seed is the first-generation seed from fields planted with breeder seed between the 37 and 44 parallels. Certified seed is the production from fields planted with either foundation or breeder seed. No other seed class or generation is to be certified.

5. 1970.

C.H. Hanson, Chairman

National Certified Alfalfa

Variety Review Board

Members of the Board Golden Stoker

D.F. Beard

Robert Teweles

C.C. Lowe

Alternates of the Board

Frank G. Parsons

E.H. Beyer

Eldon Olson

E.H. Stanford