1. The selection criteria used in the development of this variety include forage yield, forage quality, persistence and resistance to one or more of the following pests: bacterial wilt, Fusarium wilt, Verticillium wilt, anthracnose (Race 1), Phytophthora root rot, and Aphanomyces root rot (Race 1 and Race 2). Parent plants contain tolerance to Roundup® (glyphosate) herbicide conferred by the CP4 5-enolpyruvylshikimate-3-phosphate synthase (cp4-epsps) transgene, specifically, the USDA deregulated Roundup Ready® alfalfa transgenic events J101 and/or J163 (OECD unique identifiers: MON-00101-8 and MON-00163-7, respectively).

2. This variety is adapted to the North Central and East Central regions. This variety has been tested in Wisconsin, Indiana, Pennsylvania and Iowa, and is intended for use in the North Central and East Central regions.

3. Test variety is Moderately Dormant, similar to FD4 check. Test variety is Very Winterhardy, similar to WS2 check. Flower color (Syn2) is 95% purple, 4% variegated and 1% cream with a trace of yellow and white. This variety has high multifoliolate leaf expression. Test variety is “Roundup Ready®” expressing tolerance to Roundup® herbicide conferred by the cp4-epsps transgene.

4. Test variety has high resistance to anthracnose (Race 1), bacterial wilt, Phytophthora root rot, Verticillium wilt, Fusarium wilt and Aphanomyces root rot (Race 1); resistance to root-knot nematode (M. hapla) and pea aphid; with moderate resistance to stem nematode and spotted alfalfa aphid. Reaction to blue alfalfa aphid has not been tested.

5. Seed increase is on a limited generation basis with one generation each of breeder and foundation and two generations of certified seed classes. Breeder (Syn 1), foundation (Syn 2), and certified (Syn 2 or Syn 3) classes will be recognized. Production of Syn 2 foundation seed requires consent of the breeder. Breeder seed was produced in the field near Nampa, ID in 2003. Forage Genetics will maintain sufficient foundation seed for the projected life of the variety. Stands of foundation and certified seed fields are limited to 3 and 6 years, respectively. At least one glyphosate application is required during early stand establishment so that cp4-epsps null segregant plants are removed from the seed field prior to pollination. (Null segregant plants are the plants that do not contain the Roundup Ready® trait due to normal genetic segregation in this variety).

6. Certified seed will be marketed in 2006. 7. No decision has been made concerning Plant Variety Protection Act.

8. The information in this application may not be forwarded to the PVP office.

9. Variety Name: RRALF 4R100 Date submitted November 21, 2005

Experimental designations: FG R43M706

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