1. DS288 is a three clone 75-95% hybrid alfalfa variety. Parent clones were selected out of forage yield plots and/or disease nurseries. These parent plants were tested for male sterility, maintaining and restoration ability. The parent plants were selected for one or more of the following traits: forage yield, stand persistence, forage quality, resistance to: Phytophtora root rot, anthracnose (Race 1) and Verticillium wilt.

2. DS288 is adapted to the Southwest Region of the United States and intended for use across the Southwest Region of the United States. The state where it has been tested is California.

3. DS288 is a non-dormant variety similar to the fall dormancy 8 check. Flower color of the male line in the Syn. 2 generation is 100% purple, with trace amounts of variegated, cream and white. Flower color of the female line in the F1 generation is 100% purple, with trace amounts of variegated, cream and white.

4. DS288 has high resistance to anthracnose (Race 1), stem nematode, northern root-knot nematode (M. hapla); resistance to Phytophtora root rot and southern root knot nematode (M. incognita) and moderate resistance to pea aphid. DS288 has not been tested for resistance to blue alfalfa aphid, spotted alfalfa aphid, Aphanomyces root rot (Race1), bacterial wilt, Fusarium wilt and Verticillium wilt.

5. Female Breeder seed was produced by crossing the cytoplasmic male sterile line (A) by the maintainer line (B) in field isolation near Sloughhouse, CA in 2000-2002. Female seed was kept separate across production years. Male Breeder seed (Syn. 1) was produced in field isolation near Sloughhouse in 2000.

Male Foundation seed (Syn. 2) was produced from Breeder seed. Hybrid seed (F1) was produced from crossing female seed by either Breeder or Foundation male seed. Two generations of male seed are recognized. A maximum of three harvest years each is permitted on stands producing Breeder and Foundation seed with five years for Certified seed. Dairyland Research International will maintain sufficient seed for the projected life of the variety.

6. Certified Seed will be available fall of 2006.

7. Application for the Plant Variety Protection is undecided.

8. Information in the NAVRB application can be forwarded to the PVP office.

9. Variety name:

Date Submitted November 23, 2005

Experimental designations: DS288

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