1. This cultivar is a broad based germplasm pool developed by four cycles of among and within half-sib family selection from within a breeding population designated UC-356. UC-356 was developed from nine different source pools in the University of California alfalfa breeding program. The component populations had previously been selected for resistance to saline soil conditions, root knot nematode (Meloidogyne sp.), Phytophthora root rot (Phytophthora megasperma), bacterial wilt (Clavibactor insidiosum), and Fusarium root rot (Fusarium oxysporum), blue alfalfa aphid (Acyrthosiphon kondoi), Pea aphid (Acyrthosiphon pisum), spotted alfalfa aphid (Threioaphis maculata), and forage yield and adaptation in the low desert production area of California and Arizona. The primary selection criterion in developing this cultivar were adaptation to low desert production conditions, resistance to and the silverleaf whitefly (Bemisia argentifolii) and high seed yield. This germplasm pool is composed of: 0%, M. falcata: 0%, Ladak; 1%, M. vana; 8%, Turkistan; 0%, Flemish; 7%, Chilean; 1%, Peruvian; 15%, Indian; 35%, African; 10%, Arabian; and 23%, unknown sources of germplasm.

2. This cultivar is adapted to Low Desert irrigated production areas. It has been tested in the Imperial San Joaquin Valley of California, and Central Arizona. It is intended for hay, haylage, greenchop, or dehydration. The target market area will be the Low Desert irrigated alfalfa production areas of California and Extreme South Western Arizona.

3. This cultivar is very nondormant (Group 9) with a Fall Dormancy rating of 8.7 based on University of California Dormancy Trials, Flower color is predominantly purple (>98%) with a trace of Variegated types ( 1%) and a trace of Cream (< 1%). Flower color data were determined on Syn 4 (UC-2681).

4. It is highly resistant to Fusarium wilt (Fusarium oxysporum) and spotted alfalfa aphid (Threioaphis maculata). It is resistant to Phytophthora root rot (Phytopithora megasperma), blue alfalfa aphid (Acyrthosiphon kondoi, pea aphid (Acyrthosiphon pisum), and southern root knot nematode (Meloidogyne incognita). It is moderately resistant to bacterial wilt (Clavibactor insidiosum) and has low resistance to southern anthracnose (Colletotrichum trifolii) (Race 1) and the Silverleaf Whitefly (Bemisia argentifolii). Resistance of this cultivar to Verticillium wilt (Verticillium albo-atrum) and Aphanomyces root rot (Race 1) (Aphanomyces euteiches) and stem nematode (Ditylenchus dispasaci) is unknown

5. Seed classes of this cultivar will be Breeder (produced in a field isolation in 1998), Foundation, and Certified. The University of California Foundation Seed Project, Davis or its designee, will maintain breeder and Foundation seed classes. Foundation and Certified seed production are each limited to a 3-year stand life. Seed production of both Foundation and Certified classes is limited to the San Joaquin Valley of California south of 37 25'N latitude and Riverside and Imperial counties of California south of 34 00'N latitude.

6. If approved for certification, Certified seed will first be offered for sale in 2000.

7. Application for Plant Variety Protection will be made. Request for the certification option will be made.

8. As a means of added varietal protection, are you willing to have the information in this application turned over to the PVP office? YES

9. Variety name: UC-Impalo-WF Date Submitted: December 1, 2002

Experimental Designations: UC-2458 (pre-breeder, Syn l), UC-2531 (breeder, Syn 2), UC-2598 (foundation, Syn 3), UC-2681 (certified, Syn 4)

Date NA & MLVRB first accepted this variety: December 2000

Dates previous amendments were accepted: None

Date this amendment submitted: December 1, 2002

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