August, 1998
Potato Leafhopper Resistance
Empoasca fabae (Harris)
Mark McCaslin and David Miller
PLANT CULTURE

Greenhouse

Container ........ Bench, flat, or pot deep enough to allow root development
Media ............. sand, soil or potting mix
Temp/light ...... 24 to 30oC; 16+ hr daylength
No. of Plants ...25+ per replication
No. of Reps..... 3 to 6 replications
Other .............. Spray and fertilize as necessary

FIELD ESTABLISHMENT

Location ......... Transplant 8-12 week old plants to the field in mid to late May.
Spacing .......... 0.3 to 0.4m x 0.6 to 1.0m
Culture ........... Maintain vigorous growth
and control weeds.
Location ......... Test sites should be limited to areas where potato leafhopper populations normally cause damage.


RATING

Rating can be taken at any time during the
establishment year when the susceptible check is showing severe and uniform stunting and
yellowing.

A 1-5 scale is used to rate PLH damage. Both foliar discoloration (yellowing and/or reddening) and plant stunting are used to assess damage.

1 ........ No apparent injury
2 ........ Very minor stunting and yellowing
3 ........ Moderate stunting, yellowing is evident on 20-40% of leaves
4 ........ Significant injury. Plants showing significant stunting with yellowing on 40-60% of leaves.
5 ........ Severe injury. Plants with severe stunting; yellowing or reddening evident of 60-100% of leaves.

Alfalfa populations can be characterized for resistance to PLH by calculating an average severity index (ASI) and percentage of resistant plants (i.e. combined percentage of plants rated as class 1 and 2.)
CHECK CULTIVARS
  Approximate
Expected
Resistance(%)
Acceptable
Range of
Resistance(%)
Resistant    
PLH25 25 15 - 40
Susceptible    
Ranger 0 0 - 1


DISTRIBUTION AND SEVERITY OF POTATO LEAFHOPPER

Potato Leafhopper Empoasca fabae Harris
Click on the map above for a larger version. See also the KEY.

SCIENTISTS WITH EXPERTISE

Name ....... Mark McCaslin
Address .... Forage Genetics
N5292 Gills Coulee Rd
West Salem, WI 54669
Phone .......(608) 786-2121

Name ....... Dave Miller
Address .... Pioneer HiBred Intl.
W8131 Hwy 60
Arlington, WI 53911
Phone ....... (608) 635-7396

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